Staff Scientist II will utilize their Molecular Biology experience and knowledge to work with a cross-functional team of cell and molecular biologists within the Gene Editing Institute. The successful candidate is expected to carry out the assigned projects mostly independently. Primary responsibilities include but are not limited to developing, optimizing, executing and analyzing in-silico, in-vitro and in-vivo CRISPR off-target assays for gene therapy products.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Design and optimize PCR reactions
Restriction digestion, conventional and seamless cloning
Perform total RNA and genomic DNA purification from mammalian cells
Carry out Sanger and next generation sequencing (NGS)
Close communication with all involved team members and supervisors
Maintenance of laboratory equipment
Maintenance of a highly organized, technical and detailed laboratory notebook containing protocols used, results derived from each experiment or measurement
Preparation and dissemination of technical reports
Present research findings, including preparation of research reports and abstracts, dissemination at national and international scientific conferences and publication of manuscripts by peer-reviewed scientific journals.
Maintain familiarity with current scientific literature and techniques in the field of CRISPR based gene therapy
Perform assigned work safely, adhering to established departmental safety rules and practices; report to Group leader, in a timely manner, any unsafe activities, conditions, hazards, or safety violations that may cause injury to oneself, other employees, patients and visitors.
Performs other related duties as required/assigned.
SCOPE, PURPOSE, AND FREQUENCY OF CONTACTS:
Scientific opinions and discussion to collaborators.
DIRECTION/SUPERVISION OF OTHERS:
Limited supervision of laboratory assistants
Group leader / Director of Gene Editing Institute
EDUCATION AND EXPERIENCE REQUIREMENTS:
Bachelor's degree in molecular biology, biochemistry, biological sciences or related field with 3 - 7 years of work experience or MS with 0 - 4 years of work experience
Extensive experience with wide variety of molecular biology techniques
Experience with Sanger and Next-generation sequencing and analysis is a plus
Experience in fast-paced biotechnology industry is preferred
Experience in cell and molecular biology assay development
Experience with managing multiple projects simultaneously
KNOWLEDGE, SKILL, AND ABILITY REQUIREMENTS:
Strong knowledge and skill base of molecular biology methods
Well-versed in culturing mammalian cells is a plus
Strong troubleshooting skills
Adaptable to incorporating new methods to improve various aspects of workflows
Ability to effectively work independently and as part of a team
Skilled in effective communication and reporting with team members and supervisors
Knowledge in CRISPR genome engineering
Experienced in in vitro assays using immortalized cancer cells
Experienced in cancer biology/genetics
Frequent sitting, standing and walking with or without reasonable accommodation for physical disabilities
Office, clinical and laboratory settings
Internal Number: JR55518
About ChristianaCare Health System
ChristianaCare Health System is headquartered in Wilmington, Delaware and is one of the country's largest health care providers, ranking 21st in the nation for hospital admissions. Christiana Care is proudly a Nurse Magnet recognized institution. Christiana Care Health System is also one of the largest health care providers in the mid-Atlantic region, serving all of Delaware and portions of seven counties bordering the state in Pennsylvania, Maryland and New Jersey. A not-for-profit, non-sectarian health system, Christiana Care includes two hospitals with more than 1,100 patient beds, and is a major teaching hospital with two campuses. Christiana Care is continually recognized for excellence on a regional and national level. Our role in the community is expressed in the Christiana Care Way: "We serve our neighbors as respectful, expert, caring partners in their health. We do this by creating innovative, effective, affordable systems of care that our neighbors value."Christiana Care is a great place to work because we value diversity and recognize it to be a core part of our success. Because of the diversity of our employees, affiliated health professionals and volunteers, we are ...positioned to meet the unique needs of our patients and community. We acknowledge and celebrate the uniqueness and talent of each employee. Because of our talented workforce we are able to provide a quality healthcare experience to our patients and community. We strive to create an inclusive environment in which individual diversity can be leveraged and thrive. Christiana Care Health System is an equal opportunity employer, firmly committed to prohibiting discrimination, whose staff is reflective of its community and considers qualified applicants for open positions without regard to race, color, sex, religion, national origin, sexual orientation, genetic information, gender identity or expression, age, veteran status, disability, pregnancy, citizenship status, or any other characteristic protected under applicable federal, state, or local law.